KMID : 0545120130230111627
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Journal of Microbiology and Biotechnology 2013 Volume.23 No. 11 p.1627 ~ p.1635
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Mitochondria-Targeted Apoptosis in Human Cytomegalovirus-Infected Cells
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Lee Gyu-Cheol
Lee Jae-Ho Kim Bo-Yeon Lee Chan-Hee
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Abstract
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Mitochondria often play central roles in apoptotic pathways, and disruption of the mitochondrial transmembrane potential (¥Ä¥×m) has been observed in various cells undergoing apoptosis. Human cytomegalovirus (HCMV) infection induces apoptosis in permissive cells; however, investigations of mitochondria-targeted apoptosis in HCMV-infected human foreskin fibroblast (HFF) cells have been limited. Here, we investigated the mitochondrial apoptosis pathway in HCMV-infected HFF cells. Flow cytometry analysis using JC-1 revealed that HCMV infection induces disruption of ¥Ä¥×m in HFF cells when administered 24 h postinfection (hpi), and this disruption was maximized at 48 hpi. Moreover, cytochrome c, normally a mitochondrial inner membrane protein, was detected in cytoplasmic extracts of HCMV-infected cells, but not mock-infected cells, by western blot analysis at 24 hpi. A caspase activity assay based on fluorescence spectrophotometry using a fluorogenic substrate revealed an increase in caspase-3 activity at 48 hpi in HCMV-infected cells. Caspase-8 activity was increased at 72 hpi in HCMV-infected cells. These results imply that HCMV infection induces mitochondria-mediated apoptosis in HFF cells.
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KEYWORD
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apoptosis, caspase, cytomegalovirus, mitochondria
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